Details

Nucleic Acids as Molecular Diagnostics


Nucleic Acids as Molecular Diagnostics


1. Aufl.

von: Andreas Keller, Eckart Meese

106,99 €

Verlag: Wiley-VCH
Format: PDF
Veröffentl.: 04.09.2014
ISBN/EAN: 9783527672233
Sprache: englisch
Anzahl Seiten: 392

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Beschreibungen

By integrating technology, supporting infrastructure and efficient application, the all-in-one guide presents molecular diagnostics as an essential component of modern, personalized clinical practice. It considers all important aspects, from the hardware and software needed, to recent improvements in blood- and non-blood-based biomarker tests. Chapters on ethical challenges and a look at current trends and the latest innovations are also included.<br> Bridging the gap between industry and academia, this is a highly useful resource for practitioners as well as for developers of modern, DNA- and RNA-based molecular diagnostics.
Preface<br> <br> NEXT-GENERATION SEQUENCING FOR CLINICAL DIAGNOSTICS OF CARDIOMYOPATHIES<br> Introduction<br> Cardiomyopathies and Why Genetic Testing is Needed<br> NGS<br> NGS for Cardiomyopathies<br> Sample Preparation<br> Bioinformatics Analysis Pipeline<br> Interpretation of Results and Translation into Clinical Practice<br> <br> MICRORNAs AS NOVEL BIOMARKERS IN CARDIOVASCULAR MEDICINE<br> Introduction<br> miRNAs are Associated with Cardiovascular Risk Factors<br> miRNAs in Coronary Artery Disease<br> miRNAs in Cardiac Ischemia and Necrosis<br> miRNAs as Biomarkers of Heart Failure<br> Future Challenges<br> <br> MICRORNAs IN PRIMARY BRAIN TUMORS: FUNCTIONAL IMPACT AND POTENTIAL USE FOR DIAGNOSTIC PURPOSES<br> Background<br> Gliomas<br> Meningiomas<br> Pituitary Adenomas<br> Medulloblastomas<br> Other Brain Tumors<br> Summary and Outlook<br> <br> GENETIC AND EPIGENETIC ALTERATIONS IN SPORADIC COLORECTAL CANCER: CLINICAL IMPLICATIONS<br> Introduction<br> Microsatellite Instability<br> Driver Somatic Mutations in CRC<br> Epigenetic Instability in CRC<br> Hypomethylation<br> CpG Island Methylator Phenotype<br> Concluding Remarks<br> <br> NUCLEIC ACID-BASED MARKERS IN UROLOGIC MALIGNANCIES<br> Introduction<br> Bladder Cancer<br> Prostate Cancer<br> Renal Cell Carcinoma<br> Summary<br> <br> FROM THE GENETIC MAKE-UP TO THE MOLECULAR SIGNATURE OF NON-CODING RNA IN BREAST CANCER<br> Introduction<br> Molecular Breast Cancer Detection<br> Molecular Breast Cancer Subtypes and Prognostic/Predictive Molecular Biomarkers<br> <br> NUCLEIC ACID-BASED DIAGNOSTICS IN GYNECOLOGICAL MALIGNANCIES<br> Introduction<br> Cervix, Vulva, and Vaginal Carcinoma<br> Endometrial Carcinoma (Carcinoma Corpus Uteri)<br> Ovarian Carcinoma<br> Breast Cancer<br> Conclusion<br> <br> NUCLEIC ACIDS AS MOLECULAR DIAGNOSTICS IN HEMATOPOIETIC MALIGNANCIES -<br> IMPLICATIONS IN DIAGNOSIS, PROGNOSIS, AND THERAPEUTIC MANAGEMENT<br> Introduction<br> Methodological Approaches<br> Cytogenetic Analysis to Molecular Diagnostics<br> Minimal Residual Disease<br> Chronic Myeloid Leukemia<br> Acute Myeloid Leukemia<br> Acute Lymphocytic Leukemia<br> Chronic Lymphocytic Leukemia<br> Outlook and Perspectives<br> <br> TECHNIQUES OF NUCLEIC ACID-BASED DIAGNOSIS IN THE MANAGEMENT OF BACTERIAL AND VIRAL INFECTIOUS DISEASES<br> Importance of Nucleic Acid-Based Molecular Assays in Clinical Microbiology<br> Nucleic Acid Amplification Techniques<br> Post-Amplification Analyses<br> General Overview and Concluding Remarks<br> <br> MICRORNAs IN HUMAN MICROBIAL INFECTIONS AND DISEASE OUTCOMES<br> Introduction<br> General Aspects of miRNAs in Infectious Diseases<br> miRNAs as Biomarkers and Therapeutic Agents in Tuberculosis and Hepatitis C Infections<br> miRNA-Targeting Therapeutics<br> Concluding Remarks<br> <br> TOWARDS THE IDENTIFICATION OF CONDITION-SPECIFIC MICROBIAL POPULATIONS FROM HUMAN METAGENOMIC DATA<br> Introduction<br> Nucleic Acid-Based Methods in Diagnostic Microbiology<br> Need for Comprehensive Microbiome Characterization in Medical Diagnostics<br> Challenges for Metagenomics-Based Diagnostics: Read Lengths, Sequencing Library Sizes, and Microbial Community Composition<br> Deconvolution of Population-Level Genomic Complements from Metagenomic Data<br> Need for Comparative Metagenomic Data Analysis Tools<br> Future Perspectives in Microbiome-Enabled Diagnostics<br> <br> GENOME, EXOME, AND GENE PANEL SEQUENCING IN A CLINICAL SETTING<br> Introduction<br> Genetic Diagnostics from a Laboratory Perspective -<br> From Sanger to NGS<br> NGS Diagnostics in a Clinical Setting -<br> Comparison Between Genome, Exome, and Panel Diagnostics<br> Conclusion and Outlook<br> <br> ANALYSIS OF NUCLEIC ACIDS IN SINGLE CELLS<br> Introduction<br> Isolating Single Cells<br> Looking at the DNA of a Single Cancer Cell<br> Molecular DNA Analysis in Single Cells<br> Approaches to Analyze RNA of a Single Cell<br> Expression Analysis in Single Cells and its Biological Relevance in Cancer<br> Thoughts on Bioinformatics Approaches<br> Future Impact of Single-Cell Analysis in Clinical Diagnosis<br> <br> DETECTING DYSREGULATED PROCESSES AND PATHWAYS<br> Introduction<br> Measuring and Normalizing Expression Profiles<br> Biological Networks<br> Measuring the Degree of Deregulation of Individual Genes<br> Over-Representation Analysis and Gene Set Enrichment Analysis<br> Detecting Deregulated Networks and Pathways<br> miRNA Expression Data<br> Differential Network Analysis<br> Conclusion<br> <br> COMPANION DIAGNOSTICS AND BEYOND -<br> AN ESSENTIAL ELEMENT IN THE PUZZLE OF TRANSFORMING HEALTHCARE<br> Introduction<br> The Healthcare Environment<br> What is Companion Diagnostics?<br> What are the Drivers for Companion Diagnostics?<br> Companion Diagnostics Market<br> Partnerships and Business Models for Companion Diagnostics<br> Regulatory Environment for Compantion Diagnostics Tests<br> Outlook -<br> Beyond Companion Diagnostics Towards Holistic Solutions<br> <br> ETHICAL, LEGAL, AND PSYCHOSOCIAL ASPECTS OF MOLECULAR GENETIC DIAGNOSIS<br> General Peculiarities of Genetic Diagnoses<br> Informed Consent and Genetic Counseling<br> Medical Secrecy and Data Protection<br> Predictive Diagnosis<br> Prenatal Diagnosis<br> Multiparameter Testing<br> <br> Index
Andreas Keller studied Computational Biology at Saarland University in Saarbrucken (Germany), completing his PhD in 2009. He joined Febit Biomed GmbH in 2008 where he directed the biomarker discovery effort. In 2011, he joined the healthcare division of Siemens AG in Erlangen as director diagnostic innovation. In 2013 he became professor for Clinical Computational Biology at Saarland University. Dr. Keller has published more than 80 peer-reviewed manuscripts and field more than 30 patents in the area of biomarker discovery and molecular diagnostics.<br> <br> Eckart Meese is Professor of Human Genetics and Molecular Biology at Saarland University Medical School in Homburg (Germany), where he directs the Institute of Human Genetics. He obtained his PhD in biology in 1987 and did postdoc work at the Arizona Cancer Center in Tucson (USA). He then joined the University of Michigan Medical Center as Assistant Professor, before joining Saarland University in 1992. His scientific focus is on miRNA and autoantibody profiling for the diagnosis of cancer and other diseases.<br>
By integrating technology, supporting infrastructure and efficient application, this all-in-one guide presents molecular diagnostics as an essential component of modern, personalized clinical practice. It considers all important aspects, from the hardware and software needed, to recent improvements in blood- and non-bloodbased biomarker tests. Chapters on ethical challenges and a look at current trends and the latest innovations are also included.<br /><br />Bridging the gap between industry and academia, this is a highly useful resource for practitioners as well as for developers of modern, DNA- and RNA-based molecular diagnostics.

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